Immunochemical characterization of rough Brucella lipopolysaccharides
نویسندگان
چکیده
منابع مشابه
Immunochemical characterization of rough Brucella lipopolysaccharides.
Lipopolysaccharides (LPS) were extracted from rough strains of Brucella abortus and Brucella melitensis and from strains of the naturally occurring rough species Brucella ovis and Brucella canis. Brucella rough lipopolysaccharides (R-LPS) were readily distinguished from Brucella smooth lipopolysaccharides (S-LPS) and enterobacterial R-LPS, by their chemical, physical, and serological characteri...
متن کاملImmunochemical characterization of Brucella lipopolysaccharides and polysaccharides.
Purified lipopolysaccharide (LPS) extracted with phenol-water from smooth Brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees C. The degraded polysaccharide (AH) released gave reactions of identity with the native polysaccharide hapten (NH) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of B. abortus and with the polysaccharide (poly B) extracted by tri...
متن کاملComparison of Biological and Immunological Characterization of Lipopolysaccharides From Brucella abortus RB51 and S19
BACKGROUND Brucella abortus RB51 is a rough stable mutant strain, which has been widely used as a live vaccine for prevention of brucellosis in cattle instead of B. abortus strain S19. B. abortus lipopolysaccharide (LPS) has unique properties in comparison to other bacterial LPS. OBJECTIVES In the current study, two types of LPS, smooth (S-LPS) and rough (R-LPS) were purified from B. abortus ...
متن کاملCapsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterization.
Fermentor growth of Bacteroides fragilis under controlled conditions in a complex medium containing 1% glucose and 10% fetal calf serum resulted in high yields of bacteria. After hot phenol-water extraction of the organisms, capsular polysaccharide was isolated from the aqueous phase and purified by Sephacryl S-300 chromatography in a buffer with 3% sodium deoxycholate. Lipopolysaccharide was i...
متن کاملcomparison of biological and immunological characterization of lipopolysaccharides from brucella abortus rb51 and s19
conclusions purified lps can be considered as a safe adjuvant and can be used as a component in prophylactic and therapeutic vaccines targeting infectious disease, cancer and allergies. results biochemical analysis data and sds-page profile showed that the chemical nature of s19 lps is different from rb51 lps. both s and r-lps induce an immune response. t-cell immune response induced by both s ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1984
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.43.3.779-782.1984